Minghua Jiang, Weihua Pan, Amir Arastehfar, Wenjie Fang, Liyan ling, Hua Fang, Farnaz Farnaz Daneshnia, Jian Yu, Wanqing Liao, Hao Pei, Xiaojing Li, Cornelia Lass-Florl
medRxiv 2020.03.15.20036376; doi: https://doi.org/10.1101/2020.03.15.20036376

Smith College

RT-LAMP functions as using multiple loop primers target the ORF1ab gene and the S gene used for DNA strand displacement activity and then target amplification is used. This method has reported a 100% sensitivity and 100% specificity, and the mean detection time was under 30 minutes. Additionally, RT-LAMP assay was two times faster than qRT-PCR and is storable at room temperature. This permits labs to extend their capacity and process two times more clinical samples. The technique could potentially be used for high-throughput screening purposes. 

RT-LAMP

Development and validation of a rapid single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable and high-throughput screening of COVID-19

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