The energy payoff phase of glycolysis involves the conversion of the 2 molecules of G3P from the investment phase into 2 molecules of pyruvate, during which 4 ATP and 2 NADH are also generated.

University of Notre Dame

The two phases of the EMP pathway are:

- Energy Investment Phase
- Energy Payoff Phase

Two Phases of the EMP Pathway

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The energy investment phase of glycolysis involves the conversion of 6-carbon glucose into 2 molecules of 3-carbon glyceraldehyde 3-phosphate (G3P), using 2 ATP.

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