A positive enzyme immunoassay (EIA) result is indicated by a color change, occurring when a substrate reacts with the enzyme attached to antibodies bound to trapped viral antigens. A negative result displays no color change because the unbound antibody-enzyme conjugate is washed away before substrate addition.

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The mechanism of an enzyme immunoassay (EIA) involves conjugating a colorless enzyme tag to a detecting antibody. When the antibody binds to a target antigen (often immobilized on a membrane filter) and unbound antibodies are washed away, the enzyme interacts with an added colorless substrate to produce a colored end product.

Mechanism of Enzyme Immunoassay

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Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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