The mechanism of an enzyme immunoassay (EIA) involves conjugating a colorless enzyme tag to a detecting antibody. When the antibody binds to a target antigen (often immobilized on a membrane filter) and unbound antibodies are washed away, the enzyme interacts with an added colorless substrate to produce a colored end product.

Mechanism of Enzyme Immunoassay

Enzyme immunoassays (EIAs) targeting viral antigens are commonly employed as preliminary screening tests. Due to their role as initial screens, positive results must be confirmed using assays with higher sensitivity, such as a Western blot or a nucleic acid amplification test (NAAT).

Enzyme Immunoassay as a Screening Test

An enzyme immunoassay (EIA) is a diagnostic test that utilizes the specific binding of antibodies to detect and attach to target antigens within a complex mixture of biomolecules.

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A serological assay is a diagnostic test performed on a patient's serum sample to detect the presence of specific types of viruses.

Serological Assay

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Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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