The eukaryotic ribosome is the 80S ribosome. The ribosomes have a 40S small subunit and 60S large subunit. Ribosomes in eukaryotic cells can be found in the membrane or free in the cytoplasm.

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Ribosomes are made up of RNA and proteins. They are responsible for translating mRNA into proteins. Ribosomes are present in the cytoplasm.

Ribosomes

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The A-site of the ribosome interacts with the charged tRNA which contains the amino acid bound to adenosine monophosphate (AMP).

Aminoacyl site (A-site)

The peptidyl site (P-site) of the ribosome binds the charged tRNA where the amino acid has formed a peptide bond with the growing protein, but has not severed the bond with the tRNA. 

Peptidyl site (P-site)

The exit site (E-site) is where the tRNA dissociates from the ribosome to be charged again.

Exit site (E-site)

The *E. coli* ribosome has been well studied and is the model for prokaryotic transcription. The *E. coli* ribosome has two subunits: the 30S and the 50S. In translation initiation, the 30S recognizes the Shine-Delgarno sequence and then the 50S subunit can interact with the 30S to form the assembled ribosome for translation.

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