(A) Sequence depth of reads remapped to Pangolin-CoV.

(B) Similarity plot based on the full-length genome sequence of Pangolin-CoV. Full-length genome sequences of SARS-CoV-2 (Beta-CoV/WuhanHu-1), BatCoV RaTG13, bat SARSr-CoV 21, bat SARSr-CoV45, bat SARSr-CoV WIV1, and SARSCoV BJ01 were used as reference sequences.

(C) Comparison of common genome organization similarity among SARS-CoV-2, Pangolin-CoV, and BatCoV RaTG13.

University of Michigan - Ann Arbor

- The study confirmed likeliness of SARS-CoV-2 presence in pangolins using pangolin lung samples from Liu’s study. By performing de novo assembly on 1,882 clean reads in a lung sample that covered 76.02% of the SARS-CoV-2 genome, they found that 22 out of 36 contigs best matched to SARS-CoV-2.
- A pangolin draft genome was created using a reference-guided scaffolding approach based on 34 contigs. There was a 99.99% genome coverage when comparing the draft genome to 1,882 clean reads, demonstrating that the draft genome can be reliably used for further analysis. Pangolin-CoV genome was found to be 91.02% identical to SARS-CoV-2 and 90.55% identical to RaTG13 using Simplot analysis. 

Pangolin-CoV is 91.02% identical to SARS-CoV-2 at the whole-genome level

Zhang, T., Wu, Q., & Zhang, Z. (2020). Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak. Current biology : CB, 30(7), 1346–1351.e2. https://doi.org/10.1016/j.cub.2020.03.022

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