Concept
In vitro testing procedure
- 96 well plates with 5.105 cells/mL of Vero E6 were prepared and incubated overnight at 37 degrees Celsius in a carbon dioxide atmosphere
- 1, 2, and 5 μM of HCQ were tested with 5 and 10μM of AZM
- Each test was completed at least three times and was repeated twice (5 μM of HCQ with 5 and 10 μM of AZM was repeated three times)
- Cell culture supernatant was extracted and replaced by drugs diluted in the culture medium four hours prior to infection
- At time zero virus suspension in culture medium was added to all wells except in controls where medium was added instead
- Multiplicity of infection was 0.25
- RT-PCR was performed 30 min after infection in one plate and again at 60 hours after infection on a second plate
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Updated 2020-07-28
Tags
SARS-CoV-2 (COVID-19)
Biomedical Sciences