Many plasmids contain multiple cloning sites that contain many restriction enzyme sites that are used to insert DNA fragments into the vector plasmid DNA. 

University of Wisconsin - Madison

Important Features of Vector Plasmids used for Genetic Engineering

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

OpenStax Microbiology Textbook

Multiple Cloning Sites

Reporter genes are used to distinguish between the host cells that contain the vector plasmid. It may code for a protein. 

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