126 (72.4%) of the 174 child participants had a positive bacterial culture, and cutting the tip of the NP swab did not reduce species diversity on swab microbiome.

Syracuse University

Cultures were incubated overnight at 37 °C with 5% CO2, and if no growth was present in a culture after this time, it was reincubated for another 24 hours. Researchers used standard techniques (assessment of growth in 0, 1, 2, 3, or 4 quadrants) to measure the presence of pathogenic bacteria such as Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis. Growth in 3+ and 4+ quadrants was identified as heavy growth.


Method for Testing Bacterial Culture with NP Swab

Lopez, S.M.C., Martin, J.M., Johnson, M. et al. A method of processing nasopharyngeal swabs to enable multiple testing. Pediatr Res 86, 651–654 (2019). https://doi-org.libezproxy2.syr.edu/10.1038/s41390-019-0498-1
https://www.nature.com/articles/s41390-019-0498-1

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