Method of inoculation where the liquid contents of a test tube are spread evenly over a growth medium. 

Serial dilution is a method of direct microbial growth measurement that dilutes an original culture in order to obtain a smaller, more countable number in order to calculate the  colony-forming units per milliliter (CFU/mL). The goal is to obtain CFUs between 30-300, and then proceeding to either the pour or spread plate methods of inoculation. 

Serial Dilution 

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

OpenStax Microbiology Textbook

Method of inoculation where the liquid contents of a test tube are poured and swirled around on a growth medium. 

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