Sample preparation for two-photon microscopy is fundamentally similar to the preparation used for fluorescence microscopy, with the key exception being the use of infrared dyes instead of standard fluorochromes.

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A two-photon microscope is an advanced instrument that uses a scanning technique, fluorochromes, and long-wavelength light (such as infrared) to visualize specimens. Because the long-wavelength excitation light has low energy, two photons must strike a location simultaneously to excite the fluorochrome. This low-energy light is less damaging to living cells, and the long wavelengths penetrate deep into thick specimens, resolving the resolution limitations of confocal microscopes. They are exceptionally useful for examining living cells within intact tissues like brain slices, embryos, and whole animals, though their high costs (typically \$300,000 to \$500,000) restrict their use mostly to advanced clinical and research laboratories.

Two-Photon Microscopes

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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