A negative stain is a dye that colors the background rather than the biological specimen itself. By leaving the specimen unstained and producing a dark background, this technique creates a silhouette effect where light cells stand out prominently. Common examples of negative stains include India ink and nigrosin.

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An acidic stain is a dye that colors positively charged molecules and structures, such as proteins. Depending on the specific chemistry of the bacterial cell, acidic stains can function as either positive or negative stains. Examples of acidic stains include eosin, acid fuchsin, rose Bengal, and Congo red.

Acidic Stain

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Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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