• Intermediate created: Carbamoyl phosphate
• Enzyme: Carbamoyl Phosphate Synthetase

Requires ATP

Major point of regulation

1. Activation of bicarbonate with ATP, to form carboxyphosphate

2. Addition of ammonium

3. Activation of carbamic acid with ATP to form carbamoyl phosphate

Anhydride bond, higher transfer potential

• Carbamoyl Phosphate Synthetase is responsible for substrate channeling - prevents loss of intermediates