For a sample of size m from a Normally distributed random variable with paramters μ and σ, the sample variance, which is the sum of the squared deviations from the mean over the number of samples, the estimator
E[σ′^m2]=E[m1∑i=1m(x(i)−μ^m)2]
Is a biased estimator for the variance σ2.
To see why, consider that the sample variance,
E[σ^m2]=E[m−11∑i=1m(x(i)−μ^m)2]
is an unbiased estimator for the variance σ2. Then
bias(σ^m2=E[m−11∑i=1m(x(i)−μ^m)2]−σ2=0, so E(σ^m2)=E[m−11∑i=1m(x(i)−μ^m)2]=σ2=E[m1∑i=1m(x(i)−μ^m)2]=E(σ′^m2), since m−1=m.