A majority of patients in groups II and III were receiving immunosuppression therapy. However, most patients with diagnoses of JSLE and JDM presented with persistent upregulation of interferon signaling regardless of the immunosuppressive treatment. Certain patients with sJIA did present with upregulated ISG expression even with IL1 blockers. Additionally, the interferon score should not be mistaken for other sensitive assays that can identify the disease-inducing molecule. The IS relates to the expression of ISGs and can be used, in conjunction with other technology, to describe the relationship between the inducing signal and the subsequent response.