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Maturation as a Threat to Internal Validity

Maturation is a threat to internal validity that occurs when participants undergo natural developmental or physiological changes—such as growing older, learning, or becoming fatigued—between the pretest and posttest measurements of a study. These natural, ongoing changes, rather than the experimental treatment itself, may be the actual cause of any observed differences in the dependent variable over time. For example, over the course of a year-long intervention, children naturally become better reasoners, which could be misconstrued as an effect of the intervention.

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Updated 2026-05-03

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Research Methods in Psychology - 4th American Edition @ KPU