N proteins are recruited to RTCs via binding to nonstructural protein 3 (nsp3), a component of the RTC. N proteins mainly, but not exclusively, bind to nsp3 among the other nonstructural proteins. Like every protein, nsp3 has an N- and C- terminus. N proteins interact specifically with the N-terminal region of nsp3.

N proteins interact with nonstructural protein 3

In infected cells, N proteins must be recruited to replication-transcription complexes (RTCs) for RNA synthesis to proceed. RTCs are sites of RNA synthesis and are complexes made up of nonstructural proteins (nsp). 

Wellesley College

-  N proteins are recruited to replication-transcription complexes (RTCs)

Roles of N protein in RNA synthesis

Cong, Y., Ulasli, M., Schepers, H., Mauthe, M., V'kovski, P., Kriegenburg, F., Thiel, V., de Haan, C., & Reggiori, F. (2020). Nucleocapsid Protein Recruitment to Replication-Transcription Complexes Plays a Crucial Role in Coronaviral Life Cycle. Journal of Virology, 94(4), e01925-19. 
https://doi.org/10.1128/JVI.01925-19.

Learn Before

Related

Learn After