A 5-minute nanotrap particle capture step can greatly increase the sensitivity of SARS-CoV-2 real-time RT-PCR assays. The procedure involves mixing nanotrap particles into a saliva sample and incubating it at room temperature. The nanotrap particles and viruses are then separated from the solution using a magnet, after which the particles are added to a buffer. Finally, the resulting solution undergoes real-time RT-PCR.