Phosphorescence is when photons are absorbed, and then emitted later on. 

Because microbes are small and translucent, we need to utilize other techniques to visualize the different structures, even under high levels of magnification and resolution.

Creating Contrast

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Pigments added to specimens that absorb light at specific wavelengths in order to create contrast.

Chromophores

Phosphorescence

Fluorescent dyes can absorb UV or blue light to emit different colors of visible light. 

Fluorescent Dyes

Due to refraction, different electromagnetic radiation waves can be changed into a wavelength on the visible light spectrum, allowing humans to see it. 

Electromagnetic Radiation (EMR)

Methods of preparation to allow us to view and distinguish different cells and their structures that were otherwise translucent through the use of ionic salts in stains/dyes. 

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