The study revealed successful execution of cytokine measurement, as interpreted by adequate levels of glyceraldehyde 3-phosphate dehydrogenase, a housekeeping enzyme.

Syracuse University

"Nucleic acid extraction was performed with the ABI MagMax96 Express automated instrument from one of the four aliquots prepared from the original sample. Gene expression of interleukin-6 (IL-6), IL-10, IL-13, IL-17, IL-25, and IL-33; interferon: α, β, γ, and λ; and IL-8 was measured using exon-spanning primers and probes according to the manufacturer’s instructions (TaqMan™, Applied Biosystems). All values were normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH)) housekeeping gene."

Method for Cytokine Measurement with NP Swab

Lopez, S.M.C., Martin, J.M., Johnson, M. et al. A method of processing nasopharyngeal swabs to enable multiple testing. Pediatr Res 86, 651–654 (2019). https://doi-org.libezproxy2.syr.edu/10.1038/s41390-019-0498-1
https://www.nature.com/articles/s41390-019-0498-1

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