"Nucleic acid extraction was performed with the ABI MagMax96 Express automated instrument from one of the four aliquots prepared from the original sample. Gene expression of interleukin-6 (IL-6), IL-10, IL-13, IL-17, IL-25, and IL-33; interferon: α, β, γ, and λ; and IL-8 was measured using exon-spanning primers and probes according to the manufacturer’s instructions (TaqMan™, Applied Biosystems). All values were normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH)) housekeeping gene."