"Nucleic acid extraction was performed with the ABI MagMax96 Express automated instrument and the MagMax Viral Isolation Kit. Individual real-time reverse transcriptase (RT-PCR) assays for the detection and subtyping of adenovirus, influenza subtypes A/B/C, human metapneumovirus, human rhinovirus, parainfluenza virus subtypes 1–4, and respiratory syncytial virus were performed. All specimens were tested for RNase P to confirm RNA integrity and monitor for PCR inhibitors. Samples were run in batches on an ABI Step One Plus 96-well real-time PCR instrument. Negative and positive controls were included with each run, including RNA runoff transcripts to generate a quantitation curve. Specimens were considered positive if the Ct value was <40 cycles."