121 (69.5%) of the 174 child participants tested positive for a virus, and cutting the tip of the NP swab did not affect recovery yield.

Syracuse University

"Nucleic acid extraction was performed with the ABI MagMax96 Express automated instrument and the MagMax Viral Isolation Kit. Individual real-time reverse transcriptase (RT-PCR) assays for the detection and subtyping of adenovirus, influenza subtypes A/B/C, human metapneumovirus, human rhinovirus, parainfluenza virus subtypes 1–4, and respiratory syncytial virus were performed. All specimens were tested for RNase P to confirm RNA integrity and monitor for PCR inhibitors. Samples were run in batches on an ABI Step One Plus 96-well real-time PCR instrument. Negative and positive controls were included with each run, including RNA runoff transcripts to generate a quantitation curve. Specimens were considered positive if the Ct value was <40 cycles."


Method for Viral Detection with NP Swab

Lopez, S.M.C., Martin, J.M., Johnson, M. et al. A method of processing nasopharyngeal swabs to enable multiple testing. Pediatr Res 86, 651–654 (2019). https://doi-org.libezproxy2.syr.edu/10.1038/s41390-019-0498-1
https://www.nature.com/articles/s41390-019-0498-1

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