FITC/PI Staining Patterns of HEK293T, HepG2, and Vero E6 cells based on expression of ORF3a

Rate of Apoptosis of HEK393T, HepG2, and Vero E6 cells based on expression of ORF3a

It was observed that there was greater staining in cells which the ORF3a accessory protein was expressed indicating a potential link between ORF3a and pro-apoptotic activity seen in host cells. Further evidence on top of the staining patterns was provided by the increased rate of apoptosis seen in host cells that had the ORF3a protein expressed.

Ohio State University, Columbus

The effect of the ORF3a protein on apoptosis was investigated by using an apoptosis staining method that involved double staining a culture of cells comprising of Vero E6, HepG2, and HEK293T cells with PI (propidium iodide) and FITC (annexin V-fluorescein 5-isothiocyanate (FITC) where increased staining indicates greater pro-apoptotic activity.

Apoptosis Staining Method: FITC/PI

Ren, Y., Shu, T., Wu, D. et al. The ORF3a protein of SARS-CoV-2 induces apoptosis in cells. Cell Mol Immunol 17, 881–883 (2020). https://doi.org/10.1038/s41423-020-0485-9

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