454 Sequencing is a type of next generation sequencing where a DNA sample is made into single strand fragments. The fragments are adapted to  bind to a bead. Each bead allows for isolating the DNA to be amplified by PCR and then sequenced. During sequencing probed nucleotides when annealed to DNA send a light to a detector to sequence the DNA. This allows for rapid DNA sequencing. 

University of Wisconsin - Madison

Next generation sequencing is a highly automated technology that allows for genome sequencing by creating hundreds of DNA fragments and analyzing the fragments. 

Next Generation Sequencing

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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