Cell separation occurs with cytokinesis, the division of the cytoplasm, along the previously formed septum.

The process of binary fission can be organized into three main stages: 
1) Duplication and Elongation 
2) Septum Formation 
3) Cell Separation

Stages of Binary Fission

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During the first stage of binary fission, the cell will duplicate each of its cellular components, including its circular chromosome (DNA). In order to accommodate the space needed for these new components, the cell will elongate into an oval shape.

Stage 1: Duplication and Elongation

The formation of the septum is important as it ensures that each new daughter cell will receive a copy of the full parental genome. One of each set of chromosomes is anchored to one side of cell, and a septum is formed down the middle of the elongated cell, giving the cell a place to divide.

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