Topoisomerase IV adds double double stranded breaks to the newly synthesized DNA molecules. This is needed for prokaryotic DNA replication because the DNA is concatenated, or interlinked, after replication and must be separated. 

Topoisomerase IV

Termination of replication occurs after the chromosome has been replicated. Uniquely in prokaryotes, topoisomerase IV is important in the termination of DNA replication. 
Key term in prokaryotic termination of replication:
- Topoisomerase IV

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DNA replication in bacteria occurs rapidly at approximately 1000 nucleotides per second, which is approximately ten times the speed of eukaryotes. It also occurs at a single origin to begin replication.

DNA Replication in Prokaryotes

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Initiation of DNA replication requires many proteins to interact with DNA to begin replication. Prokaryotes have a single DNA sequence to begin DNA replication. 
Important terms in prokaryotic initiation:
- Origin of replication
- Topoisomerase II
- DNA Polymerase III

Initation of DNA Replication in Prokaryotes

Termination of DNA Replication in Prokaryotes

Elongation of DNA replication requires adding the DNA bases of the elongating strands. The primers introduced in initiation are also removed. 
Key terms in prokaryotic elongation:
- DNA Polymerase I

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