RT4 cells that were pre-treated with anti-ACE2 antibodies were introduced to UFH.  100nM S1S2 was completely inhibited by UFH with an IC50 of
0.033U/ml, a measurement of the potency of an inhibitor (95% confidence interval 0.016-0.07).  This potency value is well below the serum concentrations of UFH used to treat patients prophylactically and therapeutically.  

Unfractionated Heparin (UFH) Inhibits SARS-CoV-2 S Protein Below Prophylactic and Therapeutic Serum Concentrations in RT4 Cells

RT4 cells that were pre-treated with anti-ACE2 antibodies were introduced to unfractionated heparin (UFH).  At a 10U/ml dose, 80% of 330nM S1S2 interaction with cells was inhibited (data not shown).  A significant reduction in S1S2 protein binding was observed compared to the control (p<0.01).  

Michigan State University

- unfractionated Heparin Inhibits SARS-CoV-2 S Protein Binding in RT4 Cells
- low Molecular Weight Heparins are Partial Inhibitors of SARS-CoV-2 S Protein

Heparin Studies Using RT4 Cells Pre-Treated with Anti-ACE2 Antibodies

Partridge, L. J., Urwin, L., Nicklin, M. J., James, D. C., Green, L. R., & Monk, P. N. (2020). ACE2-independent interaction of SARS-CoV-2 spike protein to human epithelial cells can be inhibited by unfractionated heparin. bioRxiv.
https://www.biorxiv.org/content/10.1101/2020.05.21.107870v2
doi: 10.1101/2020.05.21.107870

ACE2-independent interaction of SARS-CoV-2 spike protein to human epithelial cells can be inhibited by unfractionated heparin

Two low molecular weight heparins (LMWH) were introduced to RT4 cells that were pre-treated with anti-ACE2 antibodies: dalteparin and enoxaparin.  They were reported to have IC50 values, a measurement of the potency of an inhibitor, of 0.558 and 0.072U/ml.  Both were found to be partial inhibitors and less potent than unfractionated heparin.  

Learn Before

Related

Learn After