1Cademy - Antibody Binding Responses to SARS-CoV-2<br>

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Antibody Binding Responses to SARS-CoV-2

The optical density at 1:50 serum dilution was measured for 300 samples from the 65 patients. 2/65 individuals did not have a measurable antibody response, most likely due to the fact that their sera were only available up to 2- and 8-days POS. It is probable that they seroconverted at a later time point after they were discharged from the hospital (with the average seroconversion against at least 1 antigen being 12.6 days POS).
IgG responses against S, RBD, and N antigens were observed in 92.3%, 89.2% and 93.8% of individuals respectively. IgM responses against S, RBD, and N antigens were observed in 92.3%, 92.3% and 95.4% of individuals. The percentage of individuals with IgA responses to RBD and N was lower, with only 72.3% and 84.6% seropositive respectively, but the percentage for that of IgA against S was around the same as IgG and IgM.
51.6% of individuals showed synchronous seroconversion to IgG, IgM and IgA while 9.7%, 9.7%, and 9.7% of individuals showed singular seroconversion to IgG, IgM, and IgA, respectively.
58.1% of individuals showed synchronous seroconversion to S, RBD and N, whereas 16.1% of individuals showed singular seroconversion to either N or S.
Both IgM and IgA responses to all three antigens declined between 20 and 30 days POS respectively. On the other hand, the IgG response remained high even up to 94 days POS. Differences were observed with disease severity and when half maximal binding (EC50) was measured.

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Updated 2020-07-24

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