Conjugation can be used in molecular cloning experiments when a cell with the F plasmid and the recombinant DNA can mate with cells that do not have the F plasmid. Through this process, the plasmids can be transferred directly to other cells and the cell can act as a donor to other cells. 

Conjugation used in molecular cloning

During conjugation in E.coli, the conjugation pilus of the F+ cell comes into contact with the F- cell that does not contain the F plasmid. A single stranded copy of the F plasmid is transferred between the two cell. The F plasmid is then synthesized in the recipient cell to make it a F+ cell. 

University of Wisconsin - Madison

Conjugation is a mechanism of gene transfer where the cells use a conjugation pilus to transfer DNA between cells. The conjugation pilus is encoded on the F plasmid. 

Conjugation

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