Fixation is the process of securing cells to a slide. Specimens can be fixated to a slide via two mechanisms: heat or chemical. Heat fixations briefly heat a smear, while chemical utilizes chemicals such as acetic acid, ethanol, and methanol. Both methods aim to kill the microorganisms present in order to stop their movement and metabolism.

Prior to fixation, a specimen needs to be smeared onto a microscope slide. The smear ensures the sample is spread into a thin layer, allowing the different cells and structures to be viewed with little to no overlap. 

Smear Preparation for Fixation

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Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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