Because biological cells are too soft to be cut into the extremely thin sections required for a transmission electron microscope (TEM), they must be embedded in plastic resin. This involves dehydrating the cells through a series of ethanol soaks so the ethanol replaces the cellular water. The resin then dissolves in the ethanol, enters the cell, and solidifies to provide the structural support needed for cutting without damage.

Google

For a transmission electron microscope (TEM) to function properly, the specimen and the electron beam must be placed in a vacuum. Additionally, the specimen must be fully dehydrated and cut extremely thin, typically between $${}20-100$$ nm thick, to allow the electrons to successfully pass through it.

TEM Specimen Requirements

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

OpenStax Microbiology Textbook

TEM Specimen Dehydration and Embedding

An ultramicrotome is a specialized device used to cut specimens into extremely thin sections. In the preparation of samples for a transmission electron microscope (TEM), it is used to slice cells that have been dehydrated and embedded in solidified plastic resin, allowing them to be cut thinly without causing damage.

Learn Before

Related