The full-genome sequence of two SARS-CoV-2 strains were isolated from two patients diagnosed in Italy and were compared. Genome sequences were from an Italian patient and the other from a Chinese tourist visiting Rome. These were completely sequenced and analysed, after virus cultivation. Viral genome sequence of the Chinese tourist matched that of another Chinese tourist hospitalized in Rome. But the viral genome sequence from the Italian patient differed from the sequence isolated from the Chinese tourist. The strain of the Italian patient was similar with other viral strains identified in Germany and Mexico, while the strain from the Chinese tourist was similar with the Wuhan virus strain, clustered with different European strains and a strain from Australia. These results indicate how there mutations being found in the virus and similarities and differences between SARS-CoV-2 strains.