Use of oil between the objective lens and slide in order to create higher resolution by lowering the difference between refractive indexes between the lens and the glass. 


Most commonly used microscope. Produces visuals of specimen as a dark image on a light background produced by an illuminator and focused by a condenser lens. The eye pieces can be either monocular or binocular. 

Brightfield Microscopes

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The total magnification of a microscope can be calculated using the following equation:
total magnification= magnification of the lens in the eyepiece/ocular lens (usually 10x) multiplied by the magnification of the objective lens, which is rotated underneath and can have varying values. 

Calculating Magnification

Oil Immersion Lenses

- ocular lens (eye piece)   
- rotating objective lenses  
- coarse focus knob  
- fine focus knob  
- stage  
- illuminator   
- condenser  
- diaphragm  
- mechanical stage 
- x/y mechanical stage knobs  
- rheostat

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